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Histology A 10-mm long piece of the spinal column (the L2L3 vertebral body) and the surrounding muscle were collected from each sacrificed animal and fixed in 4% formaldehyde in phosphate buffer (pH 7.4)
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They found that MYC amplification promotes transcription of the E3 ligase KLHL42, leading to pRB1 ubiquitination and degradation, thereby conferring resistance to CDK4/6 inhibitors
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