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Diabetes Care 38(7):12121217
The time schedule of the device for amplification included three stages: 1- a cycle of initial denaturation at 95C for 5 min 2- forty cycles of amplification at 95C for 15 s, 60C for 15 - 20 s, and 72C for 15 - 30 s 3- a melting curve cycle at 95C for 15 s and 65C for 60 s The relative expression of the desired genes (Table 2) was performed using the CT method proposed by Livak and Schmittgen (24)
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