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coli to high cell density: A historical perspective on method development

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Biomarkers determination Several biomarkers involved in important physiological functions related to fish health status maintenance were measured, namely gill and liver superoxide dismutase (SOD) activity, gill and liver catalase (CAT) activity, gill and liver glutathione peroxidase (GPx) activity, gill and liver glutathione reductase (GR) activity, gill and liver glutathione-S-transferase (GST) activity and gill and liver lipid peroxidation (LPO) levels

The number of spermatozoa strongly bound to the zona pellucida was determined using a fluorescence microscope equipped with a 488 nm excitation laser (100 mW output)