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If there is a lack of cobalamin in the body, methylmalonyl CoA accumulates, which presumably leads to the neurological manifestations of B12 deficiency
They are typically injected into a muscle
GMP, HACCP, ISO . / FDA 7 4
The lysed tissue was centrifuged (13000 rpm, 3 min, 4 C) and the supernatant was transferred to a clean Eppendorf tube (low-bind protein) prior to protein concentration determination with the DC Protein Assay (Bio-Rad) according to manufacturers instruction
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