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For viability, 7-aminoactinomycin D (7AAD) (Thermo fisher A1310) or Zombie NIR (BioLegend 423106) diluted 1:3000 was stained concurrently with antibodies in all flow cytometry assays
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In the LIVE study, treatment with liraglutide was associated with an increase in heart rate by six BPM, as well as with a significant increase in serious cardiac side-effects (12 versus three
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