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- Ngn nga hnh thnh v pht trin melanin, ng thi tng sinh si collagen
Supernatants were assayed for total protein concentration with Bradford assay (BioRad 5000006) and 50100 g of protein was mixed with loading buffer and loaded in a 12 or 16% gradient gel (Thermo 1Fisher XP00162)
In addition, in sorting rounds 4 and 5 of the second pathway, two reactions were performed in parallel: labeling in the absence or presence of low-concentration quenchers to prevent transcellular labeling
HPII levels are expressed at high levels when cells enter stationary phase and are unaffected by hydrogen peroxide and/or anaerobiosis [9, 22]
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