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Furthermore, as mentioned (Luetic et al., 2017
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Cells were washed once with warm phenol red-free medium prior to imaging with an Evos FL Digital microscope or Incucyte S3 with 20 objectives
This adaptive mechanism is evident in the early stages of neurodegeneration, with increased expression of VEGF and other angiogenic mediators in response to hypoxic conditions (Zlokovic 2011)
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