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Sample preparation and extraction Plasma samples were thawed at 4 C and a volume of 200 L of each sample was transferred into a vial and extracted with 600 L of methanol
Anitha (Yarandi et al., 2020), and their colleagues noticed that TLR2- and TLR4-deficient mice align with the mechanism responsible for gut dysmotility in germ-free mice, characterized by a diminished quantity of nitrergic inhibitory neurons
Cell Rep
Then, inject the bacteriostatic water into the vial containing the lyophilized peptide powder
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