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Immunofluorescence against the astrocyte marker glial fibrillary acidic protein (GFAP) and the microglial marker ionized-calciumbinding adaptor molecule 1 (Iba-1) was evaluated in coronal cryosections of hippocampus (30 m thick) as previously reported (Ezquer et al., 2 , and the thickness ( z axis) was measured for each case

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