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Methods Materials Diamide, dithiothreitol (DTT), ethylenediaminetetraacetic acid (EDTA), GSH, GSSG, and yeast GR were from Sigma-Aldrich, l-cysteine was from Carl Roth, isopropyl--d-1-thiogalactopyranoside was from Serva, methyl ether poly(ethylene glycol) 24 maleimide (mmPEG) was from Iris Biotech, and NADPH was from Gerbu
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RNA libraries were constructed according to the NEBNext Ultra RNA Library Prep Kit (NEB, USA) and further sequenced on an Illumina HiSeq/MiSeq 4000 with 100 million total reads per sample (50 million paired reads)
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