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In practical terms, Melanotan I research has focused almost exclusively on pigmentation and photoprotection models, whereas Melanotan II has been utilized across pigmentation, energy homeostasis, sexual behavior, and appetite regulation studies due to its multi-receptor engagement profile

Urea buffer (2X) was added in a 1:1 ratio and samples incubated for 10 minutes at 37C prior to 10% SDS-PAGE gel separation and imaging using a ChemiDoc MP imaging system to detect fluorescently labelled cholesterol

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