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Dispose of needles in a proper sharps container
To obtain a GLP-1RG s complex with good homogeneity and stability, we used the NanoBiT tethering strategy, in which the C terminus of GLP-1R was directly attached to LgBiT subunit followed by a TEV protease cleavage site and a double MBP tag
These symptoms can overlap with and potentially worsen the gastrointestinal effects already associated with GLP-1 therapy, making it difficult to distinguish which agent is responsible for adverse effects
The structures reveal both common and unique features for the dual and triple agonism by illustrating key interactions of clinical relevance at the near-atomic level
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