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Cell and tissue cultures Cells were cultured in Eagle's minimum essential medium supplemented with 10% (v/v) fetal bovine serum, 1% non-essential amino acids, 2 mM l-glutamine, 100 U/mL penicillin and 100 mg/mL streptomycin at 37 C in a humidified atmosphere of 5% CO 2
93 In their model, cydD mutants had reduced trimethoprim uptake, which was reversed by deleting acrAB or by restoring GSH (74)
7)Referencias cientficas Pizzorno J
Feuz, M
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