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Individual hair follicles were isolated and cultured in Williams Medium E (Sigma-Aldrich), supplemented with 2 mM L-glutamine (Sigma-Aldrich), 10 g/ml insulin (Sigma-Aldrich), 100 ng/ml hydrocortisone (Sigma-Aldrich), 0.1% fungizone (Gibco), 1% antibiotic-antimycotic (Gibco), and 1% Penicillin-Streptavidin (Gibco) under standard conditions (37 C, 5% CO2)