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After 12 h incubation, cells were treated with PBS, Ru(II)-OH, NP2, or NP4 for 24 h (Ru 10 M), washed with PBS and incubated with Annexin/PI reagent in the dark for 15 min at 25 C
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Separate experiments showed the peptide preserved blood flow in ischemic tissues by influencing endothelial NOS (eNOS) activity [5]
10.3390/molecules28031155 18 CardinaliG.CeccarelliS.KovacsD.AspiteN.LottiL
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