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As control of HA2X formation, 79 DIV cultured neurons were treated with doxorubicine (0.5 M) for 24 h, fixed in 4% PFA in PBS and analyzed in immunofluorescence as described above
Oxidative stress, induced by accumulation of LPO products in the lens during the development of cataract, causes consumption of GSH and disruption of redox balance in the lens, so the age-related cataract is associated with progressive reduction of GSH concentration in the lens
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