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2014;9:e85637

Maintenance fluconazole therapy for recurrent vulvovaginal candidiasis

He said sharp objects have the potential to damage those protective barriers

However, some of the widely used affinity tags, such as the His-tag, exhibit several drawbacks and limitations, since they can increase the risk of distorting the natural conformation of the target protein or necessitate stringent elution and wash conditions affecting the yield of the target protein