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Natural GLP-1 secretion in the body follows a circadian pattern

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c) condensing Fmoc-Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)-Asp(OtBu)-Val-Ser(Oxa)-OFl (fragment-2) with peptide obtained in step-b) in presence of a coupling agent in in-situ manner, followed by deprotection in presence of base to obtain Fl-Thr(tBu)-Phe- Thr(tBu)-Ser(tBu)-Asp(OtBu)-Val-Ser(Oxa)-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly- Gln(Trt)-Ala-Ala-Lys(C18diacidmono-t-butyl-Y-Glu(AEEA-AEEA)-OtBu)-Glu(OtBu)- Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(pbf)-Gly-Arg (pbf)-Gly-OtBu