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The extracts were centrifuged at 10,000 rpm for 10 min at 4 C, and the supernatant was collected and used immediately for the assay at a dilution of 1:10 in PBS/EDTA
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This finding underscores why maintenance dosing matters and why microdosing might serve as a viable long-term strategy for people who have achieved their weight loss goals but want to prevent regain without the side effects and costs of high-dose therapy
PMID 8612489
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