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Every step, from opening the vial to withdrawing the solution, must adhere to strict sterile protocols
5 Methods 5.1 Cell culture The THP-1 human leukemic monocytic cell line, obtained from the American Type Culture Collection, was cultured in RPMI-1640 medium (R0883-500ML, Sigma-Aldrich) supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin, and incubated at 37C in a 5% CO 2 atmosphere
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Originally developed as an anti-obesity drug candidate in the late 1990s, it has since found research applications in both metabolic studies and cartilage repair investigations
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