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You will need booster shots at regular intervals to prevent the effects from fading, even though receiving this injection will prolong their duration

The total reaction mixture consisting of 1.5 ml sodium phosphate buffer (0.1 M, pH 7.5) and PVP (1% w/v) L-methionine (13 mM), enzyme extract (0.1 ml) with same amounts of NBT solution (2.25 mM), riboflavin (60 M), Na 2 CO 3 (1 M), EDTA (3 mM) and double-distilled water (1.0 ml, DDW)

Metallothionein This sulfur-rich protein binds zinc, copper and selected metals while contributing to cellular protection against oxidative stress