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It was not until a genetically engineered strain of Pseudomonas denitrificans was used, in which eight of the genes involved in the biosynthesis of the vitamin had been overexpressed, that the complete sequence of methylation and other steps could be determined, thus fully establishing all the intermediates in the pathway

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Reduced fluid intake due to nausea, combined with fluid losses from vomiting or diarrhea, can lead to volume depletion

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