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10 mg Vial Reconstitution BAC water added 2 mL Concentration 5,000 mcg/mL 250 mcg dose volume 0.05 mL = 5 units 500 mcg dose volume 0.10 mL = 10 units Doses per vial at 250 mcg 40 BAC water added 3 mL Concentration 3,333 mcg/mL 250 mcg dose volume 0.075 mL = ~7.5 units 500 mcg dose volume 0.15 mL = 15 units Doses per vial at 250 mcg 40 Units shown are U-100 insulin syringe units (where 100 units = 1.0 mL)

This method accurately identifies the characteristic ion peaks of 5amino1mq and effectively eliminates interference from excipients and impurities