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Roll the vial gently rather than shaking it, which can damage fragile peptide bonds
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Cell Culture and GSH Preparation K562, HepG2, Hela and L-02 cells were respectively cultured in RPMI 1640 (Hyclone, penicillin 100 U/mL, streptomycin 100 g/mL) adding 10% fetal bovine serum and maintained at 37 C in a humidified atmosphere including 5% CO 2 , in terms of the instructions of the American Type Culture Collection
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