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Understanding the potential causes, when to seek medical advice, and how modern healthcare can assist you is essential for peace of mind and proper care
We show delivery of proteinase K (PK), the low specificity protease also used for classical LiP, into living mammalian cells using an electroporation approach optimized to ensure rapid (millisecond) and reproducible intracellular uptake of the protease and the desired level of intracellular cleavage
Thus supplementation is often required to maintain healthy levels
This intermittent use pattern, while not extensively studied, may provide a practical approach for individuals who cannot afford or tolerate continuous long term treatment
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