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At the end point, cells were rapidly washed with ice-cold PBS and incubated at 80 C for 30 min with extraction solvent at a ratio of 1 ml per 1 10 6 cells
While a wide range of potential biomarkersincluding CA-125, various microRNAs, and immune-related moleculeshave been identified for the diagnosis and monitoring of endometriosis, their clinical utility remains limited (71)
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