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Cells were then incubated with a primary antibody for 12 h at room temperature, followed by the corresponding fluorophore-conjugated secondary antibody (Alexa Fluor) for 45 min and DAPI staining (1 g ml 1 DAPI
Discovery of the Health Benefits of Immunocal At the beginning of the 70s, under the direction of Gustavo Bounous, MD, (FRCSC), a team of researchers from the Faculty of Medicine at McGill University (Canada), was studying proteins of animal and vegetable origins in order to feed the patients with an elemental diet that would allow them to reduce their stay in hospital
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