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For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)

These further analyses revealed that among deregulated transcripts the RAC (Rho family)-alpha serine/threonine-protein kinase AKT1 resulted particularly interesting that results significantly increased in lipedema vs

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