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Immunoaffinity purification of acetylated peptides IMAC flowthrough fractions were concatenated into four fractions, reconstituted in immunoaffinity purification (IAP) buffer and incubated with anti-acetyllysine antibody beads (Cell Signaling PTMScan)
Apigenin increased production of cleaved caspase-3, cleaved caspase-8, and cleaved PARP, which inhibited the proliferation and colony formation of HER2+ BT-474 cells (160)
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