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48 h post-transfection, cells were pelleted and resuspended (~1.5 g packed volume) into 5 mL membrane preparation buffer followed by homogenisation with a polytron homogeniser at 4 C
while the T3 RNAP variant has high selectivity for the T3 promoter, it remains effectively inhibited by T7 lysozyme 23
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This piece of legislation changed health insurance access and pricing in the United States, including significant subsidies for prescription drug coverage
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