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Cells were washed twice in ice-cold PBS and lysed for 15 min on ice with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate and 0.1% SDS) supplemented with Benzonase (25 U per ml) and 1 Halt protease inhibitor cocktail

Coimmunoprecipitation experiments showed that when RAW264.7 cells were transfected with Flag-GSTP, the complex of cPKC/Flag-GSTP increased to the maximum at 5 min after LPS treatment and then maintained a high level from 15 to 30 min (Figure 6C)

The honest, current picture is more nuanced

This remarkable enhancement occurs through the VEGFR2 receptor pathway rather than by increasing VEGF-A ligand levels directly