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For ankle injuries specifically, subcutaneous injection near the affected area at 0.25 to 0.50 mg daily for 4 to 8 weeks represents the most common protocol among researchers and biohackers

This leads to increased muscle growth , density, and strength

As both an endogenous antioxidant and a regulator of mitochondrial function, melatonin uniquely bridges circadian regulation and redox homeostasis, positioning oxidative stress as a downstream consequence of circadian and melatonergic decline in aging

Results tend to be most impressive for acute soft tissue injuries less than 6 months old, while chronic structural damage may respond more slowly or incompletely