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The very low rate constants for direct, glutathione-independent dithiol-disulfide exchange reactions between glutaredoxins and roGFP2 or other protein(S 2 )/protein(SH) 2 couples (despite favorable thermodynamics) are a prerequisite and great advantage for reliable redox measurements in vivo
Longer term For longer repeated exposure, copper-related labs may be reviewed periodically with a clinician
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There are typically no immediate or uncomfortable withdrawal symptoms when you stop
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