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2 Materials and methods 2.1 Experimental design The experiment was conducted at the experimental station of the Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), in Braunschweig, Germany in accordance with the German Animal Welfare Act and permitted by the Lower Saxony State Office for Consumer Protection and Food Safety (LAVES, Oldenburg, Germany) (AZ33.19-42502-04-16/2378)