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Using 3.0mL of bacteriostatic water creates a concentration of approximately 26.7mg/mL
Whatever your protein source is, youll want to load it onto the column in pretty much the same way cap the bottom, add your interacting protein or cell lysate, cap the top, and incubate with gentle agitation for around an hour
So you no longer end up with cysteine that can be used to make glutathione
320 However, even minimally invasive BCS can result in irreversible damage
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