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Wipe the rubber stoppers with alcohol and let them dry before each pierce, use a fresh sterile needle and syringe every time, and work in a clean preparation area
Patients were compensated for sharing their experiences
Following fixation, hearts were embedded in paraffin and 5 m sections were mounted onto treated slides, and stained with hematoxylin and Eosin (H&E) and co-stained with Massons trichrome
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