When DEGs were cross-referenced to a metabolic gene list (2,921 genes) compiled from previously published studies 6,32 , we observed significant overrepresentation of metabolic DEGs, as determined by Fishers exact test (Extended Data Fig
2.1 Misfolding and aggregation of proteins In many neurodegenerative diseases, the accumulation of proteins that are misfolded plays a critical role in disease progression
After incubation with hypo-osmotic swelling solution [HOS, containing 6.75 g/L fructose and 3.67 g/L sodium citrate] at 37C for 1 h, plasma membrane integrity of each group (repeated in triplicated)was analyzed by evaluating the modifications of sperm tail to score swollen sperm according to previous publications (61)
103,104,105 The bioactive form of vitamin D (1,25(OH)2D3) also promotes the translocation of -catenin from the nucleus to the plasma membrane, represses -catenin-TCF-4 transcriptional activity, and finally activates the transcription of the DICKKOPF-1 gene, which encodes an extracellular Wnt inhibitor
