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To construct expression vectors for the secretion of Exe4, double-stranded DNAs of Exe4 fused with signal peptides (SPs) and furin-cleavable sequences were synthesized (Azenta) and inserted into a CAG promoter-containing pCAG backbone vector using the In-Fusion HD cloning kit (Takara Bio)
Participants BARI-STEP was conducted at University College London Hospitals (UCLH)
5 Conclusion Cisplatin is a highly effective and broad-spectrum anticancer drug, but its severe ototoxicity limits its clinical application, and its mechanism involves various pathways such as DNA damage, oxidative stress, inflammatory response, mitochondrial dysfunction, and ferroptosis
B vitamins also play a role in cognitive function
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