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Ascorbate can be regenerated from its oxidized forms, monodehydroascorbate (MDHA) and dehydroascorbate (DHA), by MDHA and DHA reductases (MDHARs and DHARs), respectively (Smirnoff, 2018) The activity of DHARs is coupled to oxidation of glutathione, which is also required for spontaneous, non-enzymatic reduction of DHA to ascorbate (Figure 1)
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