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If you decide to try doing it yourself, you should stick to this same timeline, as thats what has been set based on research

Tanner CM, Pahwa R, Hauser RA, Oertel WH, Isaacson SH, Jankovic J, et al

The COCs were then homogeneously distributed in 4-well plates with 500 L of maturation medium: TCM199 and 4 L/mL gentamicin, 100 M cysteamine, 10 g/mL follicle-stimulating hormone (FSH), 10 g/mL luteinizing hormone (LH), and 10% fetal calf serum (FCS)