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The immune system shifts dramatically toward Th2 dominance, flooding the skin with interleukin-4 (IL-4), interleukin-13 (IL-13), and interleukin-31 (IL-31)
Fluorescence emission readings were analyzed using Rotor-Gene Q software (Qiagen, Germany)
doi: 10.1016/bs.vh.2016.04.005
The construct incorporates an N-terminal nona-D-arginine (D-Arg)9 cell-penetrating tag fused to a segment derived from the FOXO4-p53 interaction domain, giving the peptide a strongly cationic overall character driven by its multiple arginine and lysine residues, along with a single tryptophan that supports UV monitoring at 280 nm during analytical workflows
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