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The supernatant was filtered through a 0.22 m membrane filter, and 10 L of sample was injected into an HPLC (Shimadzu (Nexera MX)) system equipped with a C18 column and a UV detector
Its prevalence has surged in the past four decades, contributing to three or more comorbidities in 60% of patients ten years after diagnosis and causing 6.7 million annual deaths
For many people, these symptoms improve as the body adjusts
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