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Dynamic glucose-stimulated insulin secretion in primary murine islets After isolation and overnight incubation in RPMI (10% foetal bovine serum (FBS), 1% penicillin/streptomycin), 75 islets were handpicked and placed into chambers containing 2.7 mM glucose KrebsRinger phosphate-HEPES (KRPH) buffer (140 mM NaCl, 4.7 mM KCl, 1.5 mM CaCl 2 , 1 mM NaH 2 PO4, 1 mM MgSO 4 , 2 mM NaHCO 3 , 5 mM HEPES and 0.1% FA-free BSA (pH 7.4)) with 100 l of Bio-Gel P-4 Media (Bio-Rad)

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Fatigue during initial adjustment is temporary and usually resolves within the first few weeks