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Cell pellets were resupended in ice-cold buffer 1 containing 20 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C and stirred on ice with DNase I and 10 mg/mL lysozyme for 1 h before sonication

Role of glutathione-S-transferases in neurological problems

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The ability to follow transcellular pathways and induce inflammation can vary greatly among meningococcal strains and, therefore, the discrepancies found in the literature between transcellular and paracellular pathways for mechanisms for crossing the nasopharyngeal barrier may be due to the use of different strains in the various studies, as well as different cellular models