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Total RNA was extracted from with Trizol reagent (Beyotime, Shanghai, China) according to the manufacturers instructions
Eur J Pediatr 159(Suppl 2):S129S135 Repetto G, del Peso A, Zurita JL (2008) Neutral red uptake assay for the estimation of cell viability/cytotoxicity
However, in the brain of neurodegenerative diseases, the excess production of ROS and NO results in neuronal damage 7,8,9
Thus, a major limitation of this study is that reported, or as yet unexplored, systemic metabolic mediators may impact tumor outcomes, therefore the findings highlight the need for additional research to explore the precise mechanisms through which GLP-receptor agonists improve tumor outcomes
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