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After treatment, the cells were washed with cold PBS and lysed in RIPA buffer (Biosesang, Republic of Korea) supplemented with a protease inhibitor cocktail (Merck, Darmstadt, Germany)
The rotarod test was employed to assess motor endurance and pain function
A.ZilkaO.AlwaseemH.LaK.YenF.et al (2020)
In order to ensure that it doesn't denature, it's best to be kept airtight before use, which is why it in produced in oral syringes
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