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Three independent experiments were carried out, and all experiments were performed in triplicate

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Following cell lysis and barcode sequencing, viability was quantified as the log 2 FC in abundance relative to untreated controls

Why It Works: PEGylation attaches polyethylene glycol to the MGF molecule, extending its half-life from just 5-7 minutes (unmodified MGF) to roughly 48-72 hours, allowing more sustained signaling Theorized to activate and proliferate skeletal muscle satellite cells, supporting local muscle repair and hypertrophy pathways tied to IGF-1 splice-variant signaling (Hill & Goldspink, 2003, J Physiol ) Proposed to aid regeneration of tissue damaged by intense mechanical stress, though this is based mainly on mechanistic, animal, and cell-model research rather than confirmed human clinical outcomes Best For: Muscle tissue repair and recovery from intense resistance training or weightlifting-related micro-injury (unapproved, research-only use case