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Step 04 Lyophilization Partially stoppered vials transfer directly into trays for lyophilization, a controlled freeze-drying cycle that sublimates water under vacuum to preserve the compound and keep it stable at room temperature

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Determine Concentration (example: 10 mg vial 1 mL water = 10 mg/mL): Concentration (mg/mL) = Total Peptide (mg)Total BAC Water (mL) Calculate Dosage Volume (example: 1 mg dose 10 mg/mL = 0.1 mL): Volume to Draw (mL) = Desired Dose (mg)Concentration (mg/mL) Convert to Syringe Units: Using a standard U-100 (1 mL/100 unit) insulin syringe, 0.1 mL equals 10 units