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Western blotting Protein lysates were extracted from frozen RV tissue or cultured cardiomyocytes using RIPA buffer supplemented with protease and phosphatase inhibitors (Beyotime, China)
Alternative pharmacological options include phentermine-topiramate (Qsymia) and naltrexone-bupropion (Contrave), though these also carry cardiac considerations
898 Lanez Lpez, M
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