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Selection depends on the model, receptor target, and endpoints defined by the study
The cells were cultured in a cell incubator at 37 C, 5% CO2, and 95% humidity
1 g each labeled cRNA was fragmented by adding 11 l 10X blocking agent (Takara Bio, Inc., Shiga, Japan and 2.2 l 25X fragmentation buffer (Takara Bio, Inc.), then heated at 60C for 30 min
It is constantly used by our body cells to keep them functioning properly and protect them from damage due to free radicals
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